INVESTIGATION OF THE ANTIEPILEPTIC EFFECTS OF WATER AND ETHANOL EXTRACTS OF ARTEMISIA ABSINTHIUM L.Melahat Hurmet ,Hulya Ozpinar ,Umit Muhammet Kocyigit
Epilepsy is a neurological condition that affects millions of people worldwide, regardless of social class, age, or race discrimination. Current medications used in the treatment of epilepsy are unable to provide a curative effect on more than twenty percent of patients, necessitating the need for additional treatment methods. Artemisia absinthium L., traditionally used for its insecticidal, antiseptic, antispasmodic, liver-inflammatory, and fever-reducing properties, is also employed in Turkish folk medicine as an antipyretic, antihelminthic, diuretic, tonic, and for relieving stomach pain. Carbonic anhydrase (CA) is a transmembrane metalloenzyme found in the structure of living organisms from the simplest to the most advanced, catalyzing the conversion of carbon dioxide to bicarbonate and containing a Zn2+ ion in its structure. CA inhibitors are used clinically as diuretics, antiglaucoma agents, and anti-epileptics. In our study, the effects of water and ethanol extracts of Artemisia absinthium L., collected in April, on carbonic anhydrase isoenzymes I and II were investigated spectrofotometrically. It was observed that both water and ethyl alcohol extracts did not significantly affect the enzyme activity. The ethyl alcohol extract of A. absinthium exhibited a higher inhibition potential compared to the water extract. However, when the in vitro enzyme activity test results were examined, it was found that these extracts did not have a significant impact on enzyme activities. This result, which is inconsistent with the literature, is thought to be due to the fact that the plant was not collected during its flowering period.
Keywords: Epilepsy, Carbonic anhydrase, CA inhibitors, Artemisia absinthium L.